Medical School of Hebei University 1 article published in JoVE Immunology and Infection Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice Ming Meng*1,2, Shengde Chen*1,2, Xiang Gao*1,2, Huifang Liu*1,2, Yuanyuan Wang*1,2, Jingnan Zhang*1,2, Haiyang Dou*1,2, Wenjuan Li*1,2, Dongzhi Chen*1,2 1Medical School of Hebei University, 2Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province This protocol uses G6PI mixed peptides to construct rheumatoid arthritis models that are closer to that of human rheumatoid arthritis in CD4+ T cells and cytokines. High purity invariant natural killer T cells (mainly iNKT2) with specific phenotypes and functions were obtained by in vivo induction and in vitro purification for adoptive immunotherapy.
