Shanghai Institutes for Biological Sciences 1 article published in JoVE Genetics Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells Huan-Huan Wei*1, Yuanlong Liu*1, Yang Wang2, Qianyun Lu1, Xuerong Yang1, Jiefu Li1, Zefeng Wang1 1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University This report describes a bioengineering method to design and construct novel Artificial Splicing Factors (ASFs) that specifically modulate the splicing of target genes in mammalian cells. This method can be further expanded to engineer various artificial factors to manipulate other aspects of RNA metabolism.