Centre for Eye and Vision Research (CEVR) 1 article published in JoVE Biology Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells Jaclyn M. L. Chang1, Junghee Seo1, Maggie Miu Yee Kwan1, Sarah Oh1, David J. McCanna1, Lakshman Subbaraman1, Lyndon Jones1,2 1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, 2Centre for Eye and Vision Research (CEVR) This article describes the procedures used to evaluate the toxicity of UV radiation and chemical toxins on a primary and immortalized cell line.