The Seventh Affiliated Hospital of Sun Yat-sen University 1 article published in JoVE Neuroscience Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System Xiaorui Wang1, Yixun Su1,2, Xuelian Hu1,3, Jianqin Niu1 1Department of Histology and Embryology, Chongqing Key Laboratory of Neurobiology, Brain, and Intelligence Research Key Laboratory of Chongqing Education Commission, Third Military Medical University, 2Research Centre, The Seventh Affiliated Hospital of Sun Yat-sen University, 3School of Medicine, Chongqing University Demyelination takes place in multiple central nervous system diseases. A reliable in vivo drug delivery technique is necessary for remyelinating drug testing. This protocol describes an osmotic pump-based method that allows long-term drug delivery directly into the brain parenchyma and improves the drug bioavailability, with broad application in remyelination research.