Nantong Maternal and Child Health Care Hospital 1 article published in JoVE Neuroscience In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos Jiaqi Wang*1,2, Rui Chai*3, Xiaoxia Fang2, Jiajia Gu4, Wanqing Xu2, Qi Chen1, Gang Chen2,3, Shunxing Zhu5, Yan Jin1 1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University This protocol describes how to obtain images by combining in situ hybridization and immunohistochemistry of zebrafish embryonic sections. In situ hybridization was performed prior to cryosectioning, followed by antibody staining. It is useful to detect the expression patterns of two genes in zebrafish if there is a paucity of antibodies.
