Hainan Institute of Zhejiang University 1 article published in JoVE Biology Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation Xiaoyuan Tao*1,2, Mengtao Gao*3, Siyuan Wang*1, Xueying Guan1,4 1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants.
