The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University 1 article published in JoVE Immunology and Infection Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice Huiqin Tang*1, Hui Xie*1, Zhun Wang1, Shuanghe Peng2, Wenli Ni1, Linpei Guo3 1Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, 2Department of Pathology, The Second Hospital of Tianjin Medical University, 3Department of Urology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.
