China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd. 1 article published in JoVE Medicine Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI Binjie Xu1,4, Lirun Chen3, Fengqi Lv3, Yuan Pan1, Xing Fu1, Zhaoqing Pei1,2 1Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Tianfu Chinese Medicine Innovation Harbour, Chengdu University of Traditional Chinese Medicine, 3School of Medical Technology, Chengdu University of Traditional Chinese Medicine, 4China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd. In this study, a series of methods are presented to prepare DESI-MSI samples from plants, and a procedure of DESI assembly installation, MSI data acquisition, and processing is described in detail. This protocol can be applied in several conditions for acquiring spatial metabolome information in plants.
