Medical School of Chinese PLA 1 article published in JoVE Medicine A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts Xiaoyi He1,2, Libing Li1, Wenhong Xu1, Shengli Jiang1,2 1Cardiovascular Surgery, First Medical Center, Chinese PLA General Hospital, 2Medical School of Chinese PLA This protocol presents a model of long-term ventricular fibrillation in rat hearts induced by continuous stimulation with low-voltage alternating current. This model has a high success rate, is stable, reliable, and reproducible, has a low impact on cardiac function, and causes only mild myocardial injury.