The Hong Kong Polytechnic University 2 articles published in JoVE Medicine A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry Jimmy Sung-Hei Tse*1, Ying-Hon Sze*1,2, Jimmy Ka-Wai Cheung1,2, King-Kit Li1, Thomas Chuen Lam1,2,3,4 1Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, 2Centre for Eye and Vision Research (CEVR), 3Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, 4Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures. Neuroscience A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid Ling-Yi Liao1,2,3, Ying-Qian Zhang4, Benson Wui-Man Lau5, Qiong Wu6, Zi-Yan Fan1,3, Qiang Gao1,3, Zhi-Hui Zhong6,7 1Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 2Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, 3Key Laboratory of Rehabilitation Medicine in Sichuan Province, West China Hospital, Sichuan University, 4Department of physiology, Southwest Medical University, 5Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, 6Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 7Sichuan Kangcheng Biotech Co., Inc. Here, we describe the procedure for a pilot study to explore the effect of repetitive transcranial magnetic stimulation with different frequencies (1 Hz/20 Hz/40 Hz) on Aβ and tau metabolism in rhesus monkey cerebrospinal fluid.
