Davidoff Center and Rabin Medical Center 3 articles published in JoVE Cancer Research Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs Neta Moskovits1,2, Ella Itzhaki1,2, Nataly Tarasenko1,2, Eva Chausky1,2, Avital Bareket-Samish3, Aleksandr Kaufman1,2, Raisa Meerson1,2, Salomon M. Stemmer1,2,4 1Felsenstein Medical Research Center, 2Davidoff Center, Rabin Medical Center, 3BioInsight Ltd., 4Sackler Faculty of Medicine, Tel Aviv University The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations. Bioengineering Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome Uri Rozovski1, Inbal Hazan-Halevy2, George Calin3, David Harris4, Ping Li4, Zhiming Liu4, Michael J. Keating4, Zeev Estrov4 1Division of Hematology, Davidoff Cancer Center, Rabin Medical Center, 2The Center of Nanoscience and Nanotechnology, Tel Aviv University, 3Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, 4Department of Leukemia, The University of Texas MD Anderson Cancer Center Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest. Medicine Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment Hadas Bar-Joseph1,2, Salomon Marcello Stemmer2,3, Ilan Tsarfaty2,4, Ruth Shalgi1,2, Irit Ben-Aharon2,3 1Department of Cell and Developmental Biology, Tel Aviv University, 2Sackler Faculty of Medicine, Tel Aviv University, 3Institute of Oncology, Davidoff Center and Rabin Medical Center, 4Department of Clinical Microbiology and Immunology, Tel Aviv University We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.
