The Hebrew University-Hadassah Medical School 3 articles published in JoVE Medicine Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes Marco Canella*1, Jianmei Tan*1,2, Bing Su2, Michal Shoshkes-Carmel1 1Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University - Hadassah Medical School, 2Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine Here, we present a protocol to isolate murine intestinal mesenchyme, including telocytes. These can be used for several applications, such as co-culture with mouse or human-derived organoids, to support growth and better reflect the situation in the original tissue. Biology Simple Continuous Glucose Monitoring in Freely Moving Mice Doron Kleiman1, Mika Littor1,2, Mahmoud Nawas3, Rachel Ben-Haroush Schyr1, Danny Ben-Zvi1 1Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, 2Department of Military Medicine and “Tzameret”, Faculty of Medicine, Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces, 3Department of Surgery, Hadassah Medical Center-Ein Kerem Campus Here, we describe a simple method to implant a commercial continuous glucose monitor designed for patients onto mice and provide the scripts to analyze the results. Cancer Research Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence Balakrishnan Solaimuthu1, Arata Hayashi1, Anees Khatib1, Yoav D. Shaul1 1Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School Here, we describe a noninvasive monitoring method involving luciferase and green fluorescent protein expression in various breast cancer cell lines. This protocol provides a technique to monitor tumor formation and metastatic colonization in real time in mice.
