Sanjay Gandhi Postgraduate Institute of Medical Sciences 1 article published in JoVE Genetics Reverse Genetics to Engineer Positive-Sense RNA Virus Variants Shalini Soni1, Shubhra Agarwal1, Rakesh Aggarwal2, Naga Suresh Veerapu1 1Virology Section, Department of Life Sciences, Shiv Nadar University, 2Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.