University School of Biotechnology, G.G.S. Indraprastha University 1 article published in JoVE Biology Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe Vaibhav Bhardwaj*1, Kumari Sweta*1, Deepak Gyala1, Nimisha Sharma1 1University School of Biotechnology, G.G.S. Indraprastha University This work describes a protocol for a multicopy suppressor genetic screen in Schizosaccharomyces pombe. This screen uses a genome-wide plasmid library to identify suppressor clone(s) of a loss-of-function phenotype associated with a query mutant strain. Novel genetic suppressors of the ell1 null mutant were identified using this screen.
