All India Institute of Medical Sciences (AIIMS) 1 article published in JoVE Medicine Protocol for Developing a Femur Osteotomy Model in Wistar Albino Rats Aayush Aryal1, Pawan Kumar Pagaku2, Devanjan Dey2, Sagar Tyagi2, Vadanya Shrivastava2, Aditi Bhattacharya2, Sweety Rani2, Debdatta Nayak6, Anil Khurana6, Puneet Khanna3, Ankur Goyal4, Asit Ranjan Mridha5, Bhavuk Garg1, Sudip Sen2 1Department of Orthopedics, All India Institute of Medical Sciences (AIIMS), 2Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 3Department of Anaesthesiology Pain Medicine and Critical Care, All India Institute of Medical Sciences (AIIMS), 4Department of Radiodiagnosis, All India Institute of Medical Sciences (AIIMS), 5Department of Pathology, All India Institute of Medical Sciences (AIIMS), 6Central Council for Research in Homoeopathy (CCRH) Here, we present a protocol to iatrogenically fracture the shaft of the femur of Wistar albino rats and follow up on the development of the callus. This femur osteotomy model can help researchers evaluate the process of fracture healing and to study how a drug could influence fracture healing.