LV Prasad Eye Institute 1 article published in JoVE Developmental Biology Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells Sudipta Mahato*1,2, Trupti Agrawal*1,2, Divya Pidishetty*1,2, Savitri Maddileti1, Vinay Kumar Pulimamidi1,3, Indumathi Mariappan1 1Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, 2Manipal Academy of Higher Education, 3Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School This protocol describes an efficient method of differentiating hiPSCs into eye field clusters and generating neuro-retinal organoids using simplified culture conditions involving both adherent and suspension culture systems. Other ocular cell types, such as the RPE and corneal epithelium, can also be isolated from mature eye fields in retinal cultures.
