Tokyo Metropolitan Institute of Medical Science 2 articles published in JoVE Neuroscience Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex Taisuke Yoneda1, Seiichiro Sakai1,2, Hisato Maruoka1, Toshihiko Hosoya1 1RIKEN Brain Science Institute, 2Tokyo Metropolitan Institute of Medical Science Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex. Neuroscience Pentylenetetrazole-Induced Kindling Mouse Model Tadayuki Shimada1, Kanato Yamagata1 1Synaptic Plasticity Project, Tokyo Metropolitan Institute of Medical Science This protocol describes a method of chemical kindling with pentylenetetrazole and provides a mouse model of epilepsy. This protocol can also be used to investigate vulnerability to seizure induction and pathogenesis after epileptic seizures in mice.
