Kurume University 1 article published in JoVE Neuroscience Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila Hiroshi Ishimoto1, Hiroko Sano2 1Division of Biological Science, Graduate School of Science, Nagoya University, 2Department of Molecular Genetics, Institute of Life Science, Kurume University This paper describes a protocol for ex vivo calcium imaging of the Drosophila brain. In this method, natural or synthetic compounds can be applied to the buffer to test their ability to activate particular neurons in the brain.