National Cerebral and Cardiovascular Center Research Institute 1 article published in JoVE Medicine Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels Daiki Seya1, Yuqing Xu2,3, Toshifumi Mukunoki4, Kenichi Tsujita3, Osamu Nakagawa1, Yuichiro Arima1,2,3,4 1Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, 2International Research Center for Medical Sciences (IRCMS), Kumamoto University, 3Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, 4X-Earth Center, Faculty of Advanced Science and Technology, Kumamoto University Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.