Tokyo Medical Center, National Hospital Organization 1 article published in JoVE Biology Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes So Goto1,2,3, Michael Frost4, Christine Wildsoet1 1Herbert Wertheim School Optometry and Vision Science, University of California, Berkeley, 2Department of Ophthalmology, Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center, National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.
