Institut Pasteur Korea 1 article published in JoVE Neuroscience Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells Honggun Lee1, Constantin Radu1, Jeung Whan Han2, Regis Grailhe3 1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, 2School of Pharmacy, Sungkyunkwan University, 3Technology Development Platform, Institut Pasteur Korea We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.
