Hanyang University 8 articles published in JoVE Chemistry Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison Sophie M. Briffa1, Jo Sullivan2, Agnieszka Siupa2, Pauline Carnell-Morris2, Michele Carboni2, Kerstin Jurkschat3, Ruud J. B. Peters4, Carolin Schultz5, Kang Hee Seol6, Sook-Jin Kwon6, Sehee Park7, Tae Hyun Yoon6,7, Colin Johnston3, Stephen Lofts8, Eugenia Valsami-Jones1 1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2Malvern Panalytical, 3Department of Materials, University of Oxford, 4Wageningen Food Safety Research, 5UK Centre for Ecology & Hydrology, 6Institute for Next Generation Material Design, Hanyang University, 7Department of Chemistry, College of Natural Sciences, Hanyang University, 8UK Centre for Ecology & Hydrology The protocol described here aims to measure the hydrodynamic diameter of spherical nanoparticles, more specifically gold nanoparticles, in aqueous media by means of Nanoparticle Tracking Analysis (NTA). The latter involves tracking the movement of particles due to Brownian motion and implementing the Stokes-Einstein equation to obtain the hydrodynamic diameter. Cancer Research Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane Moe Ishihara*1, Junhui Hu*1, Xiaoyu Zhang2, YongHyeon Choi3, Anthony Wong4, Celine Cano-Ruiz5, Rongwei Zhao6, Ping Tan7, Jonathan L. Tso1, Lily Wu1,8 1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Bioengineering, Hanyang University, 4Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 6School of Life Sciences, Beijing Normal University, 7Department of Urology, West China Hospital, Sichuan University, 8Department of Urology, David Geffen School of Medicine, University of California, Los Angeles Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. This protocol illustrates two novel animal models for the disease: the orthotopically implanted mouse model and the chicken chorioallantoic membrane model, both of which demonstrate lung metastasis resembling clinical cases. Immunology and Infection A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System Irfan Ullah1,2, Kunho Chung1, Jagadish Beloor2, Sang-Kyung Lee1, Priti Kumar2 1Department of Bioengineering and Institute of Nanoscience and Technology, Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases, Yale University School of Medicine Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in the central nervous system. Chemistry Solution-Processed "Silver-Bismuth-Iodine" Ternary Thin Films for Lead-Free Photovoltaic Absorbers Jae Taek Oh1,2, Dae-Hwan Kim1, Younghoon Kim1 1Convergence Research Center for Solar Energy, Daegu Gyeongbuk Institute of Science and Technology (DGIST), 2Department of Chemistry and Research Institute for Convergence of Basic Sciences, Hanyang University Herein, we present detailed protocols for solution-processed silver-bismuth-iodine (Ag-Bi-I) ternary semiconductor thin films fabricated on TiO2-coated transparent electrodes and their potential application as air-stable and lead-free optoelectronic devices. Behavior Measuring the Kinematics of Daily Living Movements with Motion Capture Systems in Virtual Reality Kyoungwon Seo1, Ahreum Lee1, Jieun Kim2, Hokyoung Ryu3, Hojin Choi4 1Department of Industrial Engineering, Hanyang University, 2Imagine Lab, Hanyang University, 3Department of Arts & Technology, Hanyang University, 4Department of Neurology, College of Medicine, Hanyang University We designed a virtual reality test to assess instrumental activities of daily living (IADL) with a motion capture system. We propose a detailed kinematic analysis to interpret the participant's various movements, including trajectory, moving distance, and time to completion to evaluate IADL capabilities. Chemistry Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles Byung Hyo Kim1,2, Junyoung Heo1,2, Won Chul Lee3, Jungwon Park1,2 1Center for Nanoparticle Research, Institute for Basic Science (IBS), 2School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 3Department of Mechanical Engineering, Hanyang University Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy. Biochemistry Oligopeptide Competition Assay for Phosphorylation Site Determination Min Sung Joo1, Ja Hyun Koo1, Sol-Bi Shin2, Hyungshin Yim2, Sang Geon Kim1 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University Peptide competition assays are widely used in a variety of molecular and immunological experiments. This paper describes a detailed method for an in vitro oligopeptide-competing kinase assay and the associated validation procedures, which may be useful to find specific phosphorylation sites. Engineering Testing of Nanoparticle Release from a Composite Containing Nanomaterial Using a Chamber System Gun Ho Lee1, Kang-Ho Ahn1, Il Je Yu2 1Department of Mechanical Engineering, Hanyang University, 2Institute of Nanoproduct Safety Research, Hoseo University Nanoparticle release is tested using a chamber system that includes a condensation particle counter, an optical particle counter and sampling ports to collect filter samples for microscopy analysis. The proposed chamber system can be effectively used for nanomaterial release testing with a repeatable and consistent data range.
