National Cancer Center Korea 1 article published in JoVE Cancer Research Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks Shiv Poojan1, Han-Seong Kim2, Ji-Woon Yoon1, Hye Won Sim1, Kyeong-Man Hong1 1Omics Core Lab, Research Institute, National Cancer Center, 2Department of Pathology, Inje University Ilsan Paik Hospital Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.
