Korea Atomic Energy Research Institute 3 articles published in JoVE Biology Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms Joo-Sung Kim1,2, Jihoon Lim3 1Korea Food Research Institute, 2Department of Food Biotechnology, Korea University of Science and Technology, 3Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter. Engineering An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter Ha Eun Shim*1,2, Sajid Mushtaq*1,3, Jongho Jeon1,3 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Chemistry, Kyungpook National University, 3Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology An efficient method for the rapid and ion-selective desalination of radioactive iodine in several aqueous solutions is described by using gold nanoparticles-immobilized cellulose acetate membrane filters. Chemistry An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group Jongho Jeon1,2, Ha Eun Shim1, Sajid Mushtaq1,2, Mi Hee Choi1, Sang Hyun Park1,2, Dae Seong Choi1, Beom-Su Jang1,2 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.