PWG Genetics Pte Ltd 1 article published in JoVE Medicine Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs Won-Jae Lee1,2, Ji-Sung Park1, Si-Jung Jang1, Seung-Chan Lee1, HyeonJeong Lee1, Jae-Hoon Lee1, Gyu-Jin Rho1, Sung-Lim Lee1 1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd. A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.
