University of Science and Technology of Daejeon 1 article published in JoVE Biology Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System Haseong Kim1, Kil Koang Kwon1, Wonjae Seong1, Seung-Goo Lee1,2 1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
