King Abdullah University of Science and Technology 8 articles published in JoVE Bioengineering Biofunctionalization of Magnetic Nanomaterials Nouf A. Alsharif1, Jasmeen S. Merzaban2, Jürgen Kosel1,2 1Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 2Division of Computer, Electrical and Mathematical Sciences and Engineering, King Abdullah University of Science and Technology In this work, we provide a protocol to biofunctionalize magnetic nanomaterials with antibodies for specific cell targeting. As examples, we utilize iron nanowires to target cancer cells. Chemistry Imine Metathesis by Silica-Supported Catalysts Using the Methodology of Surface Organometallic Chemistry Maha A. Aljuhani1, Jérémie D.A. Pelletier1, Jean-Marie Basset1 1KAUST Catalysis Center, Division of Physical Sciences and Engineering, King Abdullah University of Science and Technology (KAUST) A new group IV metal catalyst for imine metathesis is prepared by grafting amine metal complex onto dehydroxylated silica. Surface metal fragments are characterized using FT-IR, elemental microanalysis, and solid-state NMR spectroscopy. Further dynamic nuclear polarization surface enhanced NMR spectroscopy experiments complement the determination of the coordination sphere. Chemistry Iron Nanowire Fabrication by Nano-Porous Anodized Aluminum and its Characterization Niketan S. Patel1, David Lago-Cachón1, Hanan Mohammed1, Julián A. Moreno1, Jürgen Kosel1 1Sensing, Magnetism and Microsystems Group, Computer Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology In this work, we describe a protocol to fabricate iron nanowires, including the formation of the porous alumina membrane that is used as the template, electrodeposition into templates using electrolyte solution, and the release of the nanowires into the solution. Neuroscience A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells Corrado Calì1, Kalpana Kare1, Marco Agus2, Maria Fernanda Veloz Castillo1, Daniya Boges1, Markus Hadwiger2, Pierre Magistretti1 1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, 2Visual Computing Center, King Abdullah University of Science and Technology The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion. Biology In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells Francesco Castagnetti1,2, Elisabetta Fiacco3, Carol Imbriano4, Lucia Latella1,2 1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice. Bioengineering A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting Gabriel E. Büchel1,2, Brandon Carney1,3, Jun Tang1, Brian M. Zeglis1,3, Jörg Eppinger2, Thomas Reiner1,4 1Department of Radiology, Memorial Sloan Kettering Cancer Center, 2KAUST Catalysis Center, King Abdullah University of Science and Technology, 3Department of Chemistry, Hunter College, and PhD Program in Chemistry, Graduate Center of City University of New York, 4Department of Radiology, Weill Cornell Medical College This protocol describes a new intraoperative imaging technique that uses a ruthenium complex as a source of chemiluminescent light emission, thereby producing high signal-to-noise ratios during in vivo imaging. Intraoperative imaging is an expanding field that could revolutionize the way that surgical procedures are performed. Chemistry Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level Satoshi Habuchi1, Takuya Yamamoto2,3, Yasuyuki Tezuka3 1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 2Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 3Department of Organic and Polymeric Materials, Tokyo Institute of Technology A protocol for the synthesis and characterization of diffusive motion of cyclic polymers at the single molecule level is presented. Chemistry Synthesis of Non-uniformly Pr-doped SrTiO3 Ceramics and Their Thermoelectric Properties Arash Mehdizadeh Dehkordi1, Sriparna Bhattacharya2, Taghi Darroudi3, Xiaoyu Zeng2, Husam N. Alshareef4, Terry M. Tritt1,2 1Department of Materials Science and Engineering, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Electron Microscope Facility, Clemson University, 4Materials Science and Engineering, King Abdullah University of Science and Technology A protocol for the synthesis and processing of polycrystalline SrTiO3 ceramics doped non-uniformly with Pr is presented along with the investigation of their thermoelectric properties.
