Ministry of National Guard Health Affairs 1 article published in JoVE Bioengineering Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo Alaa T Alshareeda1, Batla Alsowayan1, Abdullah Almubarak2, Ayidah Alghuwainem1, Yasser Alshawakir2, Mohammed Alahmed2 1Stem Cell and Regenerative Medicine Department, King Abdullah International Medical Research Centre, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, 2College of Medicine, Experimental Surgery and Animal Laboratory, King Saud University Here, we present a protocol to develop an in vivo cancer model using cell sheet technology. Such a model could be very useful for the evaluation of anticancer therapeutics.