National Environmental Agency 1 article published in JoVE Biology Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes Irene Li*1, Keng Wai Mak*1, Jeslyn Wong1, Cheong Huat Tan1 1Environmental Health Institute, National Environmental Agency Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.
