National Science and Technology Development Agency (NSTDA) 1 article published in JoVE Developmental Biology Generation of Multicellular Human Primary Endometrial Organoids Alina R. Murphy*1, Teerawat Wiwatpanit*2, Zhenxiao Lu1, Batzaya Davaadelger1, J. Julie Kim1 1Department of Obstetrics and Gynecology, Northwestern University, 2National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) A protocol to generate human primary endometrial organoids that consist of epithelial and stromal cells and retain characteristics of the native endometrial tissue is presented. This protocol describes methods from uterine tissue acquisition to the histologic processing of endometrial organoids.
