King Mongkut's University of Technology North Bangkok (KMUTNB) 1 article published in JoVE Immunology and Infection Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus Theerawut Phusantisampan1, Pattarasuda Rawiwan2,3, Sri Rajiv Kumar Roy2, Malinee Sriariyanun4, Win Surachetpong2,3 1 We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.
