National Taiwan Ocean University 4 articles published in JoVE Bioengineering Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices Hui-Fang Chang1, Shih-En Chou1, Ji-Yen Cheng1,2,3,4 1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang Ming Chao Tung University, 3Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 4College of Engineering, Chang Gung University In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system. Chemistry Synthesizing Sodium Tungstate and Sodium Molybdate Microcapsules via Bacterial Mineral Excretion Pao-Hung Lin1, Ying-Tang Huang2, Fu-Wen Lin3 1Department of Electronic and Computer Engineering, National Taiwan University of Science and Technology, 2Department of Marine Biotechnology, National Kaohsiung Marine University, 3Institute of Applied English, National Taiwan Ocean University This work presents a protocol for manufacture sodium tungstate and sodium molybdate microcapsules via bacteria and their corresponding nanoparticles. Engineering A High Performance Impedance-based Platform for Evaporation Rate Detection Wei-Lung Chou1, Pee-Yew Lee2, Cheng-You Chen2, Yu-Hsin Lin3, Yung-Sheng Lin4 1Department of Safety, Health and Environmental Engineering, Hungkuang University, 2Institute of Materials Engineering, National Taiwan Ocean University, 3Instrument Technology Research Center, National Applied Research Laboratories, 4Department of Chemical Engineering, National United University This paper presents an impedance-based apparatus for evaporation rate detection of solutions. It offers clear advantages over a conventional weight loss approach: a fast response, high-sensitivity detection, a small sample requirement, multiple sample measurements, and easy disassembly for cleaning and reuse purposes. Bioengineering Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip Hsien-San Hou1, Hui-Fang Chang1, Ji-Yen Cheng1,2,3,4,5 1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang-Ming University, 3Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 4Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 5Ph.D. Program in Microbial Genomics, National Chung Hsing University Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.