2 articles published in JoVE
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers Huijuan You*1, Shimin Le*2,3, Hu Chen2,4, Linyan Qin1, Jie Yan2,4,5 1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 2Mechanobiology Institute, National University of Singapore, 3Department of Physics, National University of Singapore, 4Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, Xiamen University, 5Center for Bioimaging Sciences, National University of Singapore A single-molecule magnetic tweezers platform to manipulate G-quadruplexes is reported, which allows for the study of G4 stability and regulation by various proteins.
Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites Sittiporn Pattaradilokrat1, Jian Li1,2, Xin-zhuan Su1 1National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2School of Life Science, Xiamen University Genetic crosses of rodent malaria parasites are performed by feeding two genetically distinct parasites to mosquitoes. Recombinant progeny are cloned from mouse blood after allowing mosquitoes to bite infected mice. This video shows how to produce genetic crosses of Plasmodium yoelii and is applicable to other rodent malaria parasites.