3 articles published in JoVE
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila Lifen Jiang*1,2, Yinpeng Zhan*2,3, Yan Zhu2,3 1School of Life Science, University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood Ke Yang*1,2,3, Jiandong Wu*3,4, Ling Zhu1, Yong Liu1, Michael Zhang5, Francis Lin3,4,6,7 1Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 2University of Science and Technology of China, 3Department of Physics and Astronomy, University of Manitoba, 4Department of Biosystems Engineering, University of Manitoba, 5Seven Oaks General Hospital, 6Department of Immunology, University of Manitoba, 7Department of Biological Sciences, University of Manitoba This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip.
Using the optokinetic response to study visual function of zebrafish Su-Qi Zou1, Wu Yin1, Ming-Jing Zhang1, Chun-Rui Hu1, Yu-Bin Huang1, Bing Hu1 1Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China (USTC) Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.