2 articles published in JoVE
Porous Silicon Microparticles for Delivery of siRNA Therapeutics Jianliang Shen*1,2, Xiaoyan Wu*1,3, Yeonju Lee1, Joy Wolfram1,4, Zhizhou Yang1, Zong-Wan Mao2, Mauro Ferrari1,5, Haifa Shen1,6 1Department of Nanomedicine, Houston Methodist Research Institute, 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, 3Pediatrics Department of Union Hospital, Huazhong University of Science and Technology, 4CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, 5Department of Medicine, Weill Cornell Medical College, 6Department of Cell and Developmental Biology, Weill Cornell Medical College Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon microparticles.
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery Jia Fan1,2, James W. Gallagher1, Hung-Jen Wu1, Matthew G. Landry1, Jason Sakamoto1, Mauro Ferrari1, Ye Hu1 1Department of Nanomedicine, The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes.