Zhejiang Ocean University 2 articles published in JoVE Biology Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells Tianming Wang1, Xu Chen1, Ke Xu1, Bing Zhang1, Dexiang Huang1, Jingwen Yang1 1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea. Biology Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy Jingwen Yang1, Yunjun Yan1, Xiaowei Xiang1, Yuchao Xu1, Naiming Zhou2, Tianming Wang1 1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.
