Capital Medical University

19 articles published in JoVE

• Medicine

  
  A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules Jingwen Xue¹, Fangliang Xing², Yang Liu³, Tengxiao Liang^{4 1}Beijing University of Chinese Medicine, ²Beijing Intelligent Entropy Science & Technology Co Ltd., ³Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, ⁴Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation.

• Cancer Research

  
  Reconstruct Human Retinoblastoma In Vitro Xiao Zhang¹, Zi-Bing Jin^{1 1}Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.

• Medicine

  
  Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice Kui Wu¹, Lin-Ling Li², Yu-Kun Li¹, Xiao-Dong Peng¹, Meng-Xia Zhang¹, Ke-Sen Liu¹, Xue-Si Wang¹, Jia-Xue Yang¹, Song-Nan Wen¹, Yan-Fei Ruan¹, Nian Liu¹, Rong Bai^{1 1}Department of Cardiology, Bejing Anzhen Hospital, Capital Medical University, ²Department of Cardiology, Bejing Chuiyangliu Hospital This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.

• Medicine

  
  Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells Xiao Zhang¹, Zi-Bing Jin^{1 1}Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory Using a self-organizing method, we develop a protocol with the addition of COCO that could significantly increase the generation of photoreceptors.

• Neuroscience

  
  Detrusor Underactivity Model in Rats by Conus Medullaris Transection Xin Zheng*¹, Menghua Wu*¹, Jian Song², Jimao Zhao^{2 1}Department of Urology, Beijing YouAn Hospital, Capital Medical University, ²Department of Urology, Beijing Friendship Hospital, Capital Medical University We present a method for establishing a detrusor underactivity model by conus medullaris transection in rats. Detrusor underactivity was successfully stimulated in these animals. The model can be used for studying urinary tract function.

• Medicine

  
  Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography Di Liu*¹, Xizhu Xu*², Yuejin Li², Jie Zhang¹, Xiaoyu Zhang¹, Qihuan Li¹, Haifeng Hou², Dong Li², Wei Wang^1,2,3, Youxin Wang^{1 1}Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, ²School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences, Edith Cowan University Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

• Medicine

  
  Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP) PeiLun Hu*¹, JiSheng Lin*¹, JunChuan Xu¹, Hai Meng¹, Nan Su¹, Yong Yang*¹, Qi Fei^{1 1}Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case study.

• Neuroscience

  
  Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure Honghui Mao*^1,2, Xiuchao Wang*^1,3, Wen Chen⁴, FengYu Liu⁵, You Wan⁵, Sanjue Hu¹, Junling Xing^{1,6 1}Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, ²Department of Toxicology, School of Public Health, ShanXi Medical University, ³Department of Psychology, Fourth Military Medical University, ⁴Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, ⁵Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, ⁶Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain.

• Neuroscience

  
  Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea Shu-Kui Yu*¹, Zheng-De Du*¹, Qing-Ling Song¹, Teng-Fei Qu¹, Yue Qi¹, Wei Xiong¹, Lu He¹, Wei Wei², Shu-Sheng Gong¹, Ke Liu^{1 1}Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, ²Department of Otology, Shengjing Hospital of China Medical University This manuscript describes an experimental protocol for evaluating the morphological characteristics and functional status of ribbon synapses in normal mice. The present model is also suitable for noise-induced and age-related cochlear synaptopathy-restricted models. The correlative results of previous mouse studies are also discussed.

• Medicine

  
  A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica Wen Cao¹, Xiaoli Zhu², Ziren Tang³, Yuguo Song^{2 1}Department of Ultrasound Medicine, Beijing Chaoyang Hospital, Capital Medical University, ²Department of Occupational Medicine & Clinical Toxicology, Beijing Chaoyang Hospital, Capital Medical University, ³Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University Here, we present a protocol to construct a pleural effusion model in rats by intratracheal instillation of polyacrylate/nanosilica.

• Environment

  
  A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans Shan Gao*¹, Weiyang Chen*², Nan Zhang¹, Chi Xu³, Haiming Jing^1,4, Wenjing Zhang^1,4, Gaochao Han^1,4, Matthew Flavel⁵, Markandeya Jois⁵, Yingxin Zeng¹, Jing-Dong J. Han³, Bo Xian³, Guojun Li^{1,4 1}Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, China, ²College of Computer Science and Technology, Qilu University of Technology(Shandong Academy of Sciences), China, ³Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China, ⁴Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, China, ⁵School of Life Sciences, La Trobe University, Australia A quantitative method has been developed to identify and predict the acute toxicity of chemicals by automatically analyzing the phenotypic profiling of Caenorhabditis elegans. This protocol describes how to treat worms with chemicals in a 384-well plate, capture videos, and quantify toxicological related phenotypes.

• Cancer Research

  
  Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad Gan-Lin Zhang¹, Yi Zhang¹, Ke-Xin Cao¹, Xiao-Min Wang^{1 1}Oncology Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University Here, we present a protocol to implant breast cancer cells into the mammary fat pad in a simple, less invasive, and easy-to-handle way, and this mouse orthotopic breast cancer model with a proper mammary fat pad environment can be used to investigate various aspects of cancer.

• Engineering

  
  Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro Zilan Xiong*¹, Shasha Zhao*², Xu Yan^{3 1}State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, ²College of Life Science and Health, Wuhan University of Science and Technology, ³Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

• Genetics

  
  Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice Jing-Ying Guo¹, Lu He¹, Teng-Fei Qu¹, Yu-Ying Liu², Ke Liu¹, Guo-Peng Wang¹, Shu-Sheng Gong^{1 1}Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, ²Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University Here we describe canalostomy procedure which allows local drug delivery into the inner ears of adult and neonatal mice through the semicircular canal with minimum damage to hearing and vestibular function. This method can be used to inoculate viral vectors, pharmaceuticals, and small molecules into the mouse inner ear.

• Immunology and Infection

  
  An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection Chongfeng Xu¹, Junhong Ai², Qingxun Zhang¹, Ting Li³, Xiaorong Wu¹, Zhengde Xie², Ziyuan Duan^{1 1}Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, ²MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, ³State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences A simple method for obtaining NK and T cell clones from CAEBV patients was developed with high efficiency, a small amount of peripheral blood, and a low-dose of IL-2.

• Developmental Biology

  
  Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis Erica L. McGrath^1,2, Junling Gao¹, Ping Wu^{1,3 1}Department of Neuroscience and Cell Biology, University of Texas Medical Branch, ²Department of Neurology, Johns Hopkins University, ³Beijing Institute for Brain Disorders, Capital Medical University This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

• Neuroscience

  
  Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells Fancheng Yan¹, Sailiang Guo², Yijie Chai², Lan Zhang³, Kegao Liu³, Qingjun Lu⁴, Ningli Wang⁴, Shuning Li^{3 1}Aier School of Ophthalmology, Central South University, Changsha, China, ²Institute of Immunology, Tsinghua University School of Medicine, Beijing, China, ³Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China, ⁴Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China Secondary degeneration of retinal ganglion cells (RGCs) occurs commonly in glaucoma. This study describes an innovative operative approach for partial optic nerve transection. The use of this space-saving operative approach extends the model's application range, and allows exploration of secondary injury mechanisms in RGCs in a new way.

• Engineering

  
  New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells Xu Yan*^1,3,4,5, Zhaozhong Meng*², Jiting Ouyang², Yajun Qiao², Fang Yuan^{1,3,4,5 1}Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, ²School of Physics, Beijing Institute of Technology, ³Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, ⁴Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, ⁵China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University A protocol for the neuroprotective application of low-dose atmospheric pressure plasma treatment on glucose deprivation-induced SH-SY5Y injuries.

• Developmental Biology

  
  Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells Yueying Li², Yong Hai¹, Jiayu Chen³, Tie Liu^{1 1}Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, ²Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, ³Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.