Nanjing Agricultural University

8 articles published in JoVE

• Biology

  
  Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation Xiaoyuan Tao*^1,2, Mengtao Gao*³, Siyuan Wang*¹, Xueying Guan^{1,4 1}College of Agriculture and Biotechnology, Zhejiang University, ²Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, ³State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, ⁴Hainan Institute of Zhejiang University Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants.

• Biochemistry

  
  Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS Changhua Zhu*¹, Xiaoye Liu*², Wenlei Wang¹, Xiaoguang Chen³, Shangyu Gao¹, Meng Qian¹, Na Yang¹, Yifeng Xu¹, Mingjia Chen^{1 1}College of Life Sciences, Nanjing Agricultural University, ²Department of Criminal Science and Technology, Nanjing Forest Police College, ³Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.

• Bioengineering

  
  A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters Yong M. Lyu¹, Yu Q. Li¹, Hui B. Song¹, Juan Guo¹, Ting Wang¹, Li Liu¹, Gabriel Yedid², Josef Voglmeir^{1 1}Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University We describe a strategy for how to use RNA samples from unreferenced Pacific oyster specimens, and evaluate the genetic material by comparison with publicly available genome data to generate a virtually sequenced cDNA library.

• Environment

  
  Preparation of Biomass-based Mesoporous Carbon with Higher Nitrogen-/Oxygen-chelating Adsorption for Cu(II) Through Microwave Pre-Pyrolysis Kunquan Li¹, Zeqing Wan^{1 1}College of Engineering, Nanjing Agricultural University Here, we present a protocol to synthesize nitrogen/oxygen dual-doped mesoporous carbon from biomass by chemical activation in different pyrolysis modes followed by modification. We demonstrate that the microwave pyrolysis benefits the subsequent modification process to simultaneously introduce more nitrogen and oxygen functional groups on the carbon.

• Immunology and Infection

  
  Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin Dechun Zhang*¹, Xianzhao Kan*², Sarah Elizabeth Huss³, Lan Jiang², Li-Qing Chen³, Yibing Hu^{4 1}Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, ²The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, ³Department of Plant Biology, University of Illinois at Urbana-Champaign, ⁴College of Resources & Environmental Sciences, Nanjing Agricultural University A method of constructing a phylogenetic tree based on sequence homology of SWEETs from eukaryotes and SemiSWEETs from prokaryotes is described. Phylogenetic analysis is a useful tool for explaining the evolutionary relatedness between homologous proteins or genes from different organism groups.

• Immunology and Infection

  
  Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model Shouming Zhang^1,2,3, Xueping Gao^1,2,3, Genhui Xiao^1,2,3,4, Chengping Lu^1,2,3, Huochun Yao^1,2,3, Hongjie Fan^1,2,3,5, Zongfu Wu^{1,2,3 1}College of Veterinary Medicine, Nanjing Agricultural University, ²Key Lab of Animal Bacteriology, Ministry of Agriculture, ³OIE Reference Lab for Swine Streptococcosis, ⁴School of Medicine, Tsinghua University, ⁵Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis.

• Biochemistry

  
  Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺ Ya-Min Du¹, Shen-Li Zheng¹, Li Liu¹, Josef Voglmeir¹, Gabriel Yedid^{2 1}Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

• Biology

  
  Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice. Cui Cao¹, Wen J. Wang¹, Ying Y. Huang¹, Hong L. Yao¹, Louis P. Conway¹, Li Liu¹, Josef Voglmeir^{1 1}Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.