South China University of Technology 9 articles published in JoVE Immunology and Infection A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia Xinrui Wang1,2, Ruizhong Zhang2, Zefeng Lin2, Ming Fu2, Yan Chen1,2 1School of Medicine, South China University of Technology, 2 We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments. Immunology and Infection A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19 Xiaofan Jia*1, Ling He*1,2, Dongmei Miao1, Caiguo Zhang1, Marian Rewers1, Liping Yu1 1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology In line with the urgent need for screening for type 1 diabetes, celiac disease, and coronavirus disease 2019, we developed a high throughput 6-Plex electrochemiluminescence assay to simultaneously detect all four islet autoantibodies, tissue transglutaminase autoantibodies, and antibodies to the receptor binding domain of severe acute respiratory syndrome coronavirus 2. Neuroscience Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity Qiangqiang Wang*1, Ju Zhang*2, Yiyi Jiang1,3, Yue Xiao1, Xiaomin Li3, Xinliang Mao3, Zebo Huang1 1Institute for Food Nutrition and Human Health, School of Food Science and Engineering, South China University of Technology, 2School of Bioscience and Bioengineering, Hebei University of Economics and Business, 3Perfect Life & Health Institute Here, we present a protocol to assess the neuroprotective activities of test compounds in Caenorhabditis elegans, including polyglutamine aggregation, neuronal death, and chemoavoidance behavior, as well as an exemplary integration of multiple phenotypes. Medicine A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases Xiaofan Jia*1,2, Ling He*1,3, Yong Gu1, Hilary High1, Liping Yu1 1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1. Developmental Biology An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo Yunyun Jiang1, Jiahao Chen1, Jin Xu2 1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration. Chemistry Synthesis of Graphene Nanofluids with Controllable Flake Size Distributions Du Baolei1, Jian Qifei1 1Department of Mechanical and Automotive Engineering, South China University of Technology A method for synthesizing graphene nanofluids with controllable flake size distributions is presented. Neuroscience Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression Weipeng Zheng*1, Xiuhang Ruan*2, Xinhua Wei3, Fangtian Xu4, Yuanping Huang1, Ning Wang1, Haoyi Chen5, YingJie Liang6, Wende Xiao6, Xin Jiang1, Shifeng Wen6 1Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, 2Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, 3Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, 4Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, 5Department of Orthopedics, Guangzhou Chest Hospital, 6Department of Orthopedics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou Medical University Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression. Bioengineering A Performance-testing Platform for a Conduction Micropump with an FR-4 Copper-clad Electrode Plate Junyuan Feng1, Zhenping Wan1, Chen Feng1, Wanyu Wen1, Yong Tang1 1School of Mechanical and Automotive Engineering, South China University of Technology This paper presents a protocol for the fabrication of a conduction micropump using symmetric planar electrodes on flame-retardant glass-reinforced epoxy (FR-4) copper-clad laminate (CCL) to test the influence of chamber dimensions on the performance of a conduction micropump. Chemistry Green and Low-cost Production of Thermally Stable and Carboxylated Cellulose Nanocrystals and Nanofibrils Using Highly Recyclable Dicarboxylic Acids Huiyang Bian1,3, Liheng Chen2,3, Ruibin Wang2,3, Junyong Zhu3 1Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, 2State Key Lab of Pulp and Paper Engineering, South China University of Technology, 3Forest Products Laboratory, U.S. Forest Service, U.S. Department of Agriculture Here we demonstrate a novel method for green and sustainable productions of highly thermally stable and carboxylated cellulose nanocrystals (CNC) and nanofibrils (CNF) using highly recyclable solid dicarboxylic acids.
