3 articles published in JoVE
Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze Huiling Tian1, Ning Ding2, Mengwei Guo1, Shun Wang1, Zidong Wang1, Hao Liu1, Jiayi Yang1, Yujie Li3, Jingyu Ren1, Jing Jiang4, Zhigang Li1 1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Guang'anmen Hospital of China Academy of Chinese Medical Science, 3The Third Affiliated Hospital of Beijng University of Chinese Medicine, 4School of Nursing, Beijing University of Chinese Medicine Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer’s Disease model mice and to assess the effect of manual acupuncture for treating them is described.
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat Dongsheng Xu*1, Jingjing Cui*1, Jia Wang1, Zhiyun Zhang1, Chen She1, Wanzhu Bai1 1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes Jing Gao*1, Xiaolu Shi*2, Hong He1, Juhong Zhang1, Ding Lin3, Guosheng Fu1, Dongwu Lai1 1Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 2Experimental Research Center, China Academy of Chinese Medical Sciences, 3Department of Cardiology, The Third Hospital of Hangzhou City Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.