Beijing Institute for Brain Disorders 2 articles published in JoVE Developmental Biology Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies Wei Zheng1,2, Zhiguo Chen1,2 1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, 2Center of Neural Injury and Repair, Beijing Institute for Brain Disorders The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment. Behavior Using Laser Doppler Imaging and Monitoring to Analyze Spinal Cord Microcirculation in Rat Yingli Jing*1,2,3,4, Fan Bai*1,2,3,4, Hui Chen1,2,3,4, Hao Dong1,2,3,4 1China Rehabilitation Research Center, 2Institute of Rehabilitation Science of China, 3Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, 4Beijing Key Laboratory of Neural Injury and Rehabilitation Here we present a combination of laser Doppler perfusion imaging (LDPI) and laser Doppler perfusion monitoring (LDPM) to measure spinal cord local blood flows and oxygen saturation (SO2), as well as a standardized procedure for introducing spinal cord trauma on rat.
