Zunyi Medical College-Zhuhai Campus 1 article published in JoVE Cancer Research Evaluating In Vitro DNA Damage Using Comet Assay Yanxin Lu1,2, Yang Liu1, Chunzhang Yang1 1Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 2Basic Medical Science Department, Zunyi Medical College-Zhuhai Campus The comet assay is an efficient method to detect DNA damage including single and double-stranded DNA breaks. We describe alkaline and neutral comet assays to measure DNA damage in cancer cells to evaluate the therapeutic effect of chemotherapy.