The Second Hospital of Jilin University Changchun 1 article published in JoVE Immunology and Infection In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model Cara L. Haymaker*1, Yared Hailemichael*1, Yi Yang2,3, Roza Nurieva2 1Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 2Department of Immunology, University of Texas MD Anderson Cancer Center, 3Department of Radiation Oncology, The Second Hospital of Jilin University The goal of this protocol is to allow for detection of in vivo antigen-specific killing of a target cell in a murine model.