3 articles published in JoVE
Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism Xinglong Wang*1, Xuesong Wu*1, Jiating He2, Xiaolin Tao1, Hongyan Li1, Gui Zhao1, Yawen Wang1, Hongyu Chen1,2 1Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, 2Chemistry and Biological Chemistry, Nanyang Technological University We report a solution-based method to synthesize substrate-bound Au nanowires. By tuning the molecular ligands used during the synthesis, the Au nanowires can be grown from various substrates with different surface properties. Au nanowire-based nanostructures can also be synthesized by adjusting the reaction parameters.
Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents Feng Pan*2, Jie Zhang*3, Yuyan Liu1, Liangsheng Lu1, Xuefeng Qiu4, Kangtai Lv5, Qipeng Zhang1 1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, Nanjing University, 2Department of Andrology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 3School of Pharmaceutical Sciences, Nanjing Tech University, 4Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, 5Department of Ultrasound, Nanjing Qixia District Maternity and Child Health Care Hospital Intracavernosal pressure recording (ICP) is an important method to evaluate the erectile function of experimental animals. Here, a detailed protocol is demonstrated for the recording procedure of ICP by catheterizing the crura penis and then electrically stimulating the cavernous nerves in rats.
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer Zhihui Xu*1, Yiyan Song*1, Huijun Jiang2, Yan Kong3, Xiaoming Li3, Jin Chen1, Yuan Wu4 1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.