2 articles published in JoVE
Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro Jinsen Lu1, Lele Wu2, Xiaoke Wang2, Jinhang Zhu2, Juan Du2, Bing Shen2 1Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University, 2School of Basic Medical Sciences, Anhui Medical University Here we present a detailed protocol for the application of rhodamine 123 to identify the mitochondrial membrane potential (MMP) and study CLIC4 knockdown-induced HN4 cell apoptosis in vitro. Under common fluorescence microscope and confocal laser scanning fluorescence microscope, the real-time change of the MMP was recorded.
Ultrasonography in Experimental Reproductive Investigations on Rats Tianjuang Wang1,2, Lidia Oltra-Rodríguez3, Nuria García-Carrillo3, Anibal Nieto4,5, Yunxia Cao*1,2,6, María L. Sánchez-Ferrer*4,5 1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, 2Institute of Reproductive Genetics, Anhui Medical University, 3Centro Experimental en Investigaciones Biomédica (CEIB), Campus Ciencias de la Salud, Universidad de Murcia, 4Department of Obstetrics and Gynecology, 'Virgen de la Arrixaca' University Clinical Hospital, 5Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 6Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs This manuscript describes the utility of ultrasound performed on female rats to design experimental models for reproductive and gynecological investigation. A step-by-step explanation of how to perform ultrasonographic evaluation is shown.