Central South University

16 articles published in JoVE

• Immunology and Infection

  
  Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis Li Yaozhen¹, Wu Kemin¹, Ji Xiaoyu¹, Ouyang Yang¹, Tang Hongying¹, Pan Baihong^{1 1}Department of Vascular Surgery, Xiangya Hospital, Central South University This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.

• Medicine

  
  In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter Zhaokai Li*^1,2, Liu Liu*¹, Zhong Wang^{1 1}Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, ²Department of Cardiovascular Medicine, Xiangya Hospital, Central South University We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sor^{tm38(CAG-GCaMP3)Hze}/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca²⁺) flux.

• Medicine

  
  Cox-Maze IV Procedure Concomitant with Valvular Surgery In Situs Inversus Dextrocardia: A Single-Center Experience in China Cheng Luo*¹, Chengming Fan*¹, Hao Zhang¹, Long Song¹, Yuhong Liu¹, Liming Liu^{1 1}Department of Cardiovascular Surgery, the Second Xiangya Hospital, Central South University We summarize the Cox-Maze IV procedure concomitant with valvular surgery performed in patients with situs inversus dextrocardia at this institution.

• Biology

  
  A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes Xinyi Liu^1,2, Si Wu^1,2, Wenqian Li^1,2, Meihong Zhang^1,2, Yan Wu^1,2, Ning Zhou³, Pa Wu^{1,2 1}Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, ²State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, ³Department of Infectious Diseases, the Second Xiangya Hospital, Central South University This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

• Immunology and Infection

  
  Isolation of Mouse Kidney-Resident CD8⁺ T cells for Flow Cytometry Analysis Wei Liao^1,2, Chaoyu Ma¹, Nu Zhang^{1 1}Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, ²Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University Following viral infection, kidney harbors a relatively large number of CD8⁺ T cells and offers an opportunity to study non-mucosal TRM cells. Here, we describe a protocol to isolate mouse kidney lymphocytes for flow cytometry analysis.

• Immunology and Infection

  
  Optimization of the Cuff Technique for Murine Heart Transplantation Yunhan Ma*^1,2, Baiyi Xie*^1,2, Helong Dai*^3,4,5, Chenxi Wang⁶, Shujiao Liu⁶, Tianshu Lan⁷, Shuangyue Xu^1,2, Guoliang Yan^1,2,6, Zhongquan Qi^{1,2,8 1}Organ Transplantation Institute, School of Medicine, Xiamen University, ²Fujian Key Laboratory of Organ and Tissue Regeneration, ³Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, ⁴Clinical Research Center for Organ Transplantation in Hunan Province, ⁵Clinical Immunology Center, Central South University, ⁶School of Medicine, Xiamen University, ⁷Xiamen Medical College, ⁸Medical College, Guangxi University We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

• Immunology and Infection

  
  Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation Hamad H. Alanazi^1,2, Li She^1,3, Xiao-Dong Li^{1 1}Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, ²Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, ³Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.

• Behavior

  
  Assessment of Sexual Behavior of Male Mice Zi-Wei Liu¹, Ning Jiang², Xue Tao², Xiao-Ping Wang¹, Xin-Min Liu², Shui-Yuan Xiao^{3 1}Department of Psychiatry, The Second Xiangya Hospital, Central South University, National Clinical Research Center for Mental Disorders and National Technology Institute on Mental Disorders, Hunan Key Laboratory of Psychiatry and Mental Health, ²Research Center for Pharmacology & Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, ³Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University This article describes how to perform sexual behavior tests in male mice.

• Cancer Research

  
  Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis Xianquan Zhan^1,2,3,4, Huanni Li⁵, Shehua Qian^1,2,3, Xiaohan Zhan^1,2,3, Na Li^{1,2,3 1}Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, ²Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, ³State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, ⁴National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, ⁵Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.

• Medicine

  
  Blood Collection Through Subclavian Vein Puncture in Mice Hui Yang*¹, Chenlu Wu*¹, Fengjiao Liu², Mi Wang¹, Pu Zou¹, Yuhu He¹, Qiming Liu¹, Qin Zhou³, Shenghua Zhou^{1 1}Department of Cardiology, The Second Xiangya Hospital, Central South University, ²Department of Cardiology, The Xiangya Hospital, Central South University, ³Department of General Surgery, The Second Xiangya Hospital, Central South University Here, we present a protocol to take blood samples from the subclavian vein of mice.

• Cancer Research

  
  Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue Guang Lei*¹, Haiyan Yang*¹, Ting Hong², Xiangyu Zhang¹, Nong Yang¹, Yongchang Zhang^{1 1}Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, ²Hunan ClinicaI Research Center in Gynecologic Cancer, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University Here, we present a protocol of elastic staining to identify elastic fibers in pT3N0M0 gastric cancer tissues on formalin-fixed, paraffin-embedded sections. Subsequently, we describe a method to determine whether tumor cells invade beyond the elastic lamina.

• Medicine

  
  Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats Hui Yang¹, Pu Zou¹, Jingyuan Chen¹, Gaoxing Shi¹, Chenlu Wu¹, Mi Wang¹, Qin Zhou², Shenghua Zhou^{1 1}Department of Cardiology, The Second Xiangya Hospital, Central South University, ²Department of General Surgery, The Second Xiangya Hospital, Central South University Here, we present a protocol to collect blood samples from the rat subclavian vein.

• Cancer Research

  
  Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome Xianquan Zhan^1,2,3,4, Yuda Huang^1,2,3, Ying Long^{1,2,3 1}Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, ²Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, ³State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, ⁴The State Key Laboratory of Medical Genetics, Central South University Here, we present a two-dimensional gel electrophoresis (2DE) coupled with mass spectrometry (MS) to separate and identify human pituitary adenoma tissue proteome, which presents a good and reproducible 2DE pattern. Many proteins are observed in each 2DE spot when analyzing complex cancer proteome with the use of high-sensitivity MS.

• Neuroscience

  
  Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells Fancheng Yan¹, Sailiang Guo², Yijie Chai², Lan Zhang³, Kegao Liu³, Qingjun Lu⁴, Ningli Wang⁴, Shuning Li^{3 1}Aier School of Ophthalmology, Central South University, Changsha, China, ²Institute of Immunology, Tsinghua University School of Medicine, Beijing, China, ³Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China, ⁴Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China Secondary degeneration of retinal ganglion cells (RGCs) occurs commonly in glaucoma. This study describes an innovative operative approach for partial optic nerve transection. The use of this space-saving operative approach extends the model's application range, and allows exploration of secondary injury mechanisms in RGCs in a new way.

• Biology

  
  Isolation and Primary Culture of Mouse Aortic Endothelial Cells Jie-Mei Wang¹, Alex F. Chen², Kezhong Zhang^{1 1}Center for Molecular Medicine and Genetics, Wayne State University, ²Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

• Medicine

  
  Technical Aspects of the Mouse Aortocaval Fistula Kota Yamamoto^1,2, Xin Li^1,3, Chang Shu³, Tetsuro Miyata², Alan Dardik^{1,4 1}The Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, ²Department of Vascular Surgery, The University of Tokyo, ³Department of Vascular Surgery, Central South University, ⁴VA Connecticut Healthcare Systems The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.