Qilu Hospital of Shandong University 5 articles published in JoVE Neuroscience Establishment of Central Cord Syndrome Model in C57BL/6J Mouse Elham Yilizati-Yilihamu Elzat*1, Xiangchuang Fan*1, Shiqing Feng1,2 1Department of Orthopaedics, Qilu Hospital of Shandong University, 2Department of Orthopaedics, Tianjin Medical University General Hospital The present protocol simulating central cord syndrome (CCS) in mice has improved repeatability and minimized operation damage to the experimental animals, avoiding disrupting the anatomical structure excessively. The strategy in this study is advantageous because it allows for research into injury mechanisms by producing consistent results. Neuroscience Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique Elham Yilizati-Yilihamu Elzat1, Xiangchuang Fan1, Zimeng Yang1, Zhongze Yuan1, Yilin Pang2, Shiqing Feng1,2 1Department of Orthopaedics, Qilu Hospital of Shandong University, 2Department of Orthopaedics, Tianjin Medical University General Hospital Minimally invasive techniques and a simple laboratory device improve the reproducibility of the spinal cord injury model by reducing operative damage to the experimental animals and allowing anatomical morphology maintenance. The method is worthwhile because the reliable results and reproducible procedure facilitate investigations of the mechanisms of disease reparation. Biology Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues Chang Liu1, Shuangshuang Wang1, Man Liu2, Fuxiang Bai1, Zhihong Chen3, Ping Wang4, Jun Mi1,5, Xunwei Wu1 1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Shijiazhuang Shimen Experimental School, 3 This paper reports that the addition of Y-27632 to TIVA medium can significantly increase the yield of melanocytes from adult skin tissues. Developmental Biology A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue Zhenan Liu1, Jie Wen1,2, Xue Leng1, Qian Zhou1, Changkuo Zhou3, Huaqiang Zhao1, Xunwei Wu1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology, Qilu Hospital of Shandong University Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells. Neuroscience Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay Wei Li1,2, Chen Zhang1, Xiulian Sun3 1Department of Neurology, Qilu Hospital of Shandong University, 2Department of Neurology, Qingdao Municipal Hospital, 3Brain Research Institute, Qilu Hospital of Shandong University This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.
