Jianghan University 1 article published in JoVE Medicine Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System Wusong Zou1, Yanqun Yang1, Ye Gu1, Pengfei Zhu1, Mingjing Zhang1, Zhong Cheng1, Xiaoyan Liu1, Yijun Yu1, Xiaohong Peng1 1Heart Center at Puai Hospital, Wuhan Puai Hospital, Jianghan University Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.