3 articles published in JoVE
Extraction of Ramie Fiber in Alkali Hydrogen Peroxide System Supported by Controlled-release Alkali Source Chaoran Meng1, Zhaoling Li1, Chaoyun Wang2, Chongwen Yu1,2, Xuerong Bi1, Siyi Wang1 1College of Textiles, Donghua University, 2Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences Presented here is a protocol for extraction of ramie fiber in alkali hydrogen peroxide system supported by controlled-release alkali source.
Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm Xianming Yang1, Pengjun Xu2, Robert I. Graham3, He Yuan1, Kongming Wu1 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 2Tobacco Research Institute, Chinese Academy of Agricultural Sciences, 3Crop and Environment Sciences, Harper Adams University Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.
A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response Ritesh Kumar*1, Shu Wei Wu*1, Arya Bagus Boedi Iswanto1, Dhinesh Kumar1, Xiao Han2, Jae-Yean Kim1 1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.