2 articles published in JoVE
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells Xiongwei Cai1,2,3, Yi Zheng1, Nancy A. Speck2 1 A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample buffer.
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells Chun Yang*1, Wei Xiong*2, Qian Qiu2, Houda Tahiri1, Carmen Gagnon1, Guoxiang Liu2, Pierre Hardy1 1Departments of Pediatrics and Pharmacology, University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital, Third Military Medical University Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.